7QF0

Crystal structure of the SARS-CoV-2 RBD in complex with the human antibody CV2.2325

7QF0 の概要

• エントリーDOI: 10.2210/pdb7qf0/pdb
• 分子名称: Spike protein S1, CV2.2325 heavy chain, CV2.2325 light chain, ... (7 entities in total)
• 機能のキーワード: neutralization, antibody, coronavirus, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 71499.75

構造登録者

Fernandez, I.,Pederzoli, R.,Rey, F.A. (登録日: 2021-12-03, 公開日: 2022-05-18, 最終更新日: 2024-10-16)

主引用文献

Planchais, C.,Fernandez, I.,Bruel, T.,de Melo, G.D.,Prot, M.,Beretta, M.,Guardado-Calvo, P.,Dufloo, J.,Molinos-Albert, L.M.,Backovic, M.,Chiaravalli, J.,Giraud, E.,Vesin, B.,Conquet, L.,Grzelak, L.,Planas, D.,Staropoli, I.,Guivel-Benhassine, F.,Hieu, T.,Boulle, M.,Cervantes-Gonzalez, M.,Ungeheuer, M.N.,Charneau, P.,van der Werf, S.,Agou, F.,Dimitrov, J.D.,Simon-Loriere, E.,Bourhy, H.,Montagutelli, X.,Rey, F.A.,Schwartz, O.,Mouquet, H.
Potent human broadly SARS-CoV-2-neutralizing IgA and IgG antibodies effective against Omicron BA.1 and BA.2.
J.Exp.Med., 219:-, 2022

PubMed Abstract: Memory B-cell and antibody responses to the SARS-CoV-2 spike protein contribute to long-term immune protection against severe COVID-19, which can also be prevented by antibody-based interventions. Here, wide SARS-CoV-2 immunoprofiling in Wuhan COVID-19 convalescents combining serological, cellular, and monoclonal antibody explorations revealed humoral immunity coordination. Detailed characterization of a hundred SARS-CoV-2 spike memory B-cell monoclonal antibodies uncovered diversity in their repertoire and antiviral functions. The latter were influenced by the targeted spike region with strong Fc-dependent effectors to the S2 subunit and potent neutralizers to the receptor-binding domain. Amongst those, Cv2.1169 and Cv2.3194 antibodies cross-neutralized SARS-CoV-2 variants of concern, including Omicron BA.1 and BA.2. Cv2.1169, isolated from a mucosa-derived IgA memory B cell demonstrated potency boost as IgA dimers and therapeutic efficacy as IgG antibodies in animal models. Structural data provided mechanistic clues to Cv2.1169 potency and breadth. Thus, potent broadly neutralizing IgA antibodies elicited in mucosal tissues can stem SARS-CoV-2 infection, and Cv2.1169 and Cv2.3194 are prime candidates for COVID-19 prevention and treatment.

PubMed: 35704748
DOI: 10.1084/jem.20220638

実験手法

X-RAY DIFFRACTION (2.3 Å)