7QEE

SN243 mutant D415N bound to para-nitrophenyl-Beta-D-glucuronide

Summary for 7QEE

Entry DOI	10.2210/pdb7qee/pdb
Descriptor	SN243, ZINC ION, SULFATE ION, ... (6 entities in total)
Functional Keywords	enzyme discovery, carbohydrate-active enzymes (cazy), protein engineering, functional metagenomics), hydrolase
Biological source	Synthetic construct
Total number of polymer chains	2
Total formula weight	166618.12
Authors	Neun, S.,Brear, P.,Campbell, E.,Omari, K.,Wagner, O.,Hyvonen, M.,Hollfelder, F. (deposition date: 2021-12-02, release date: 2022-11-16, Last modification date: 2024-01-31)
Primary citation	Neun, S.,Brear, P.,Campbell, E.,Tryfona, T.,El Omari, K.,Wagner, A.,Dupree, P.,Hyvonen, M.,Hollfelder, F. Functional metagenomic screening identifies an unexpected beta-glucuronidase. Nat.Chem.Biol., 18:1096-1103, 2022 Cited by PubMed: 35799064 DOI: 10.1038/s41589-022-01071-x PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.374 Å)

Structure validation