7QEA

Crystal structure of fluorescein-di-Beta-D-glucuronide bound to a mutant of SN243 (D415A)

Summary for 7QEA

Entry DOI	10.2210/pdb7qea/pdb
Descriptor	SN243, (2~{S},3~{S},4~{S},5~{R},6~{S})-3,4,5-tris(oxidanyl)-6-[(1~{R})-6'-oxidanyl-3-oxidanylidene-spiro[2-benzofuran-1,9'-xanthene]-3'-yl]oxy-oxane-2-carboxylic acid, ZINC ION, ... (6 entities in total)
Functional Keywords	enzyme discovery, carbohydrate-active enzymes (cazy), protein engineering, functional metagenomics), hydrolase
Biological source	Synthetic construct
Total number of polymer chains	2
Total formula weight	167691.39
Authors	Neun, S.,Brear, P.,Campbell, E.,Omari, K.,Wagner, O.,Hyvonen, M.,Hollfelder, F. (deposition date: 2021-12-01, release date: 2022-10-12, Last modification date: 2024-02-07)
Primary citation	Neun, S.,Brear, P.,Campbell, E.,Tryfona, T.,El Omari, K.,Wagner, A.,Dupree, P.,Hyvonen, M.,Hollfelder, F. Functional metagenomic screening identifies an unexpected beta-glucuronidase. Nat.Chem.Biol., 18:1096-1103, 2022 Cited by PubMed: 35799064 DOI: 10.1038/s41589-022-01071-x PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.28 Å)

Structure validation