7Q2X
Cryo-EM structure of clamped S.cerevisiae condensin-DNA complex (Form I)
Summary for 7Q2X
| Entry DOI | 10.2210/pdb7q2x/pdb |
| EMDB information | 13783 |
| Descriptor | Structural maintenance of chromosomes protein 2, Structural maintenance of chromosomes protein 4, Condensin complex subunit 2, ... (9 entities in total) |
| Functional Keywords | condensin, smc, cell cycle |
| Biological source | Saccharomyces cerevisiae S288C More |
| Total number of polymer chains | 6 |
| Total formula weight | 539173.24 |
| Authors | Lee, B.-G.,Rhodes, J.,Lowe, J. (deposition date: 2021-10-26, release date: 2022-03-23, Last modification date: 2024-11-20) |
| Primary citation | Lee, B.G.,Rhodes, J.,Lowe, J. Clamping of DNA shuts the condensin neck gate. Proc.Natl.Acad.Sci.USA, 119:e2120006119-e2120006119, 2022 Cited by PubMed Abstract: SignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex condensin. It forms and enlarges loops in DNA through loop extrusion. Our work resolves the atomic structure of a DNA-bound state of condensin in which ATP has not been hydrolyzed. The DNA is clamped within a compartment that has been reported previously in other structural maintenance of chromosomes (SMC) complexes, including Rad50, cohesin, and MukBEF. With the caveat of important differences, it means that all SMC complexes cycle through at least some similar states and undergo similar conformational changes in their head modules, while hydrolyzing ATP and translocating DNA. PubMed: 35349345DOI: 10.1073/pnas.2120006119 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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