7Q0H

Crystal structure of the receptor binding domain of SARS-CoV-2 beta variant spike glycoprotein in complex with Beta-50 and Beta-54

7Q0H の概要

• エントリーDOI: 10.2210/pdb7q0h/pdb
• 関連するPDBエントリー: 7PRY 7PRZ 7PS0 7PS1 7PS2 7PS3 7PS4 7PS5 7PS6 7PS7
• 分子名称: Beta-54 Fab heavy chain, Beta-54 Fab light chain, Beta-50 Fab light chain, ... (6 entities in total)
• 機能のキーワード: sars-cov-2 alpha variant, beta variant, gamma variant, delta variant, b.1.1.7, b.1.351, p.1, b.1.617.2, antibody, receptor-binding-domain, spike, neutralisation, viral protein/immune system, viral protein
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 119915.92

構造登録者

Zhou, D.,Ren, J.,Stuart, D.I. (登録日: 2021-10-14, 公開日: 2021-12-22, 最終更新日: 2024-10-23)

主引用文献

Liu, C.,Zhou, D.,Nutalai, R.,Duyvesteyn, H.M.E.,Tuekprakhon, A.,Ginn, H.M.,Dejnirattisai, W.,Supasa, P.,Mentzer, A.J.,Wang, B.,Case, J.B.,Zhao, Y.,Skelly, D.T.,Chen, R.E.,Johnson, S.A.,Ritter, T.G.,Mason, C.,Malik, T.,Temperton, N.,Paterson, N.G.,Williams, M.A.,Hall, D.R.,Clare, D.K.,Howe, A.,Goulder, P.J.R.,Fry, E.E.,Diamond, M.S.,Mongkolsapaya, J.,Ren, J.,Stuart, D.I.,Screaton, G.R.
The antibody response to SARS-CoV-2 Beta underscores the antigenic distance to other variants.
Cell Host Microbe, 30:53-, 2022

PubMed Abstract: Alpha-B.1.1.7, Beta-B.1.351, Gamma-P.1, and Delta-B.1.617.2 variants of SARS-CoV-2 express multiple mutations in the spike protein (S). These may alter the antigenic structure of S, causing escape from natural or vaccine-induced immunity. Beta is particularly difficult to neutralize using serum induced by early pandemic SARS-CoV-2 strains and is most antigenically separated from Delta. To understand this, we generated 674 mAbs from Beta-infected individuals and performed a detailed structure-function analysis of the 27 most potent mAbs: one binding the spike N-terminal domain (NTD), the rest the receptor-binding domain (RBD). Two of these RBD-binding mAbs recognize a neutralizing epitope conserved between SARS-CoV-1 and -2, while 18 target mutated residues in Beta: K417N, E484K, and N501Y. There is a major response to N501Y, including a public IgVH4-39 sequence, with E484K and K417N also targeted. Recognition of these key residues underscores why serum from Beta cases poorly neutralizes early pandemic and Delta viruses.

PubMed: 34921776
DOI: 10.1016/j.chom.2021.11.013

実験手法

X-RAY DIFFRACTION (3.65 Å)