7PU5

Structure of SFPQ-NONO complex

7PU5 の概要

• エントリーDOI: 10.2210/pdb7pu5/pdb
• 分子名称: Non-POU domain-containing octamer-binding protein, Splicing factor, proline- and glutamine-rich, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: dbhs, paraspeckle, nops, rrm, nuclear protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 358635.73

構造登録者

Fribourg, S. (登録日: 2021-09-28, 公開日: 2022-03-16, 最終更新日: 2024-01-31)

主引用文献

Schell, B.,Legrand, P.,Fribourg, S.
Crystal structure of SFPQ-NONO heterodimer.
Biochimie, 198:1-7, 2022

PubMed Abstract: The Drosophila behavior/human splicing (DBHS) protein family is composed of the three members SFPQ, NONO and PSPC1. These proteins share a strong sequence and structural homology within the core-structured domains forming obligate homo- and heterodimers. This feature may lead to the simultaneous existence of six different dimeric complexes that sustain their function in many cellular processes such as pre-mRNA splicing, innate immunity, transcriptional regulation. In order to perform a complete structural analysis of all possible DBHS dimers, we have solved the crystal structure of the missing DBHS heterodimer SFPQ-NONO at 3.0 Å resolution. We identify subtle changes in amino acid composition and local secondary structure of the NOPS region orientation that may modulate affinity between complexes. Interestingly this area is found mutated in aggressive skin cancers and adenocarcinomas.

PubMed: 35245601
DOI: 10.1016/j.biochi.2022.02.011

実験手法

X-RAY DIFFRACTION (2.999 Å)