7PT7

Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I

This is a non-PDB format compatible entry.

Summary for 7PT7

• Entry DOI: 10.2210/pdb7pt7/pdb
• EMDB information: 13620
• Descriptor: Undefined Mcm4 flexible N-terminal tail, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (13 entities in total)
• Functional Keywords: helicase, activation, kinase, phosphorylation, replication
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 15
• Total formula weight: 1359453.04

Authors

Saleh, A.,Noguchi, Y.,Aramayo, R.,Ivanova, M.E.,Speck, C. (deposition date: 2021-09-26, release date: 2022-06-08, Last modification date: 2024-07-17)

Primary citation

Saleh, A.,Noguchi, Y.,Aramayo, R.,Ivanova, M.E.,Stevens, K.M.,Montoya, A.,Sunidhi, S.,Carranza, N.L.,Skwark, M.J.,Speck, C.
The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
Nat Commun, 13:2915-2915, 2022

PubMed Abstract: The controlled assembly of replication forks is critical for genome stability. The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. At present, it remains poorly understood how DDK docks onto the helicase and how the kinase targets distal Mcm subunits for phosphorylation. Using cryo-electron microscopy and biochemical analysis we discovered that an interaction between the HBRCT domain of Dbf4 with Mcm2 serves as an anchoring point, which supports binding of DDK across the MCM2-7 double-hexamer interface and phosphorylation of Mcm4 on the opposite hexamer. Moreover, a rotation of DDK along its anchoring point allows phosphorylation of Mcm2 and Mcm6. In summary, our work provides fundamental insights into DDK structure, control and selective activation of the MCM2-7 helicase during DNA replication. Importantly, these insights can be exploited for development of novel DDK inhibitors.

PubMed: 35614055
DOI: 10.1038/s41467-022-30576-1

Experimental method

ELECTRON MICROSCOPY (3.8 Å)