7PPL

SHP2 catalytic domain in complex with IRS1 (625-639) phosphopeptide (pTyr-632, pSer-636)

7PPL の概要

• エントリーDOI: 10.2210/pdb7ppl/pdb
• 分子名称: Tyrosine-protein phosphatase non-receptor type 11,Tyrosine-protein phosphatase non-receptor type 11, Insulin receptor substrate 1, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: irs1, phosphatase, shp2, insulin signaling, insulin receptor substrate 1, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34897.24

構造登録者

Sok, P.,Zeke, A.,Remenyi, A. (登録日: 2021-09-14, 公開日: 2022-09-07, 最終更新日: 2024-10-23)

主引用文献

Zeke, A.,Takacs, T.,Sok, P.,Nemeth, K.,Kirsch, K.,Egri, P.,Poti, A.L.,Bento, I.,Tusnady, G.E.,Remenyi, A.
Structural insights into the pSer/pThr dependent regulation of the SHP2 tyrosine phosphatase in insulin and CD28 signaling.
Nat Commun, 13:5439-5439, 2022

PubMed Abstract: Serine/threonine phosphorylation of insulin receptor substrate (IRS) proteins is well known to modulate insulin signaling. However, the molecular details of this process have mostly been elusive. While exploring the role of phosphoserines, we have detected a direct link between Tyr-flanking Ser/Thr phosphorylation sites and regulation of specific phosphotyrosine phosphatases. Here we present a concise structural study on how the activity of SHP2 phosphatase is controlled by an asymmetric, dual phosphorylation of its substrates. The structure of SHP2 has been determined with three different substrate peptides, unveiling the versatile and highly dynamic nature of substrate recruitment. What is more, the relatively stable pre-catalytic state of SHP2 could potentially be useful for inhibitor design. Our findings not only show an unusual dependence of SHP2 catalytic activity on Ser/Thr phosphorylation sites in IRS1 and CD28, but also suggest a negative regulatory mechanism that may also apply to other tyrosine kinase pathways as well.

PubMed: 36114179
DOI: 10.1038/s41467-022-32918-5

実験手法

X-RAY DIFFRACTION (1.53 Å)