7PKN

Structure of the human CCAN deltaCT complex

Summary for 7PKN

• Entry DOI: 10.2210/pdb7pkn/pdb
• EMDB information: 13473
• Descriptor: Centromere protein H, Centromere protein U, Centromere protein R, ... (12 entities in total)
• Functional Keywords: kinetochore, chromosome segregation, centromere, cell cycle
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 11
• Total formula weight: 410976.36

Authors

Muir, K.W.,Yatskevich, S.,Bellini, D.,Barford, D. (deposition date: 2021-08-25, release date: 2022-04-27, Last modification date: 2024-07-17)

Primary citation

Yatskevich, S.,Muir, K.W.,Bellini, D.,Zhang, Z.,Yang, J.,Tischer, T.,Predin, M.,Dendooven, T.,McLaughlin, S.H.,Barford, D.
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome.
Science, 376:844-852, 2022

PubMed Abstract: Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-A, and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle.

PubMed: 35420891
DOI: 10.1126/science.abn3810

Experimental method

ELECTRON MICROSCOPY (3.2 Å)