7PHG

MaP OF P5C3RBD Interface

Summary for 7PHG

• Entry DOI: 10.2210/pdb7phg/pdb
• EMDB information: 13415
• Descriptor: Surface glycoprotein, Heavy ChaIn variable, Light ChaIn (3 entities in total)
• Functional Keywords: rbd, antibody, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); More
• Total number of polymer chains: 3
• Total formula weight: 47055.47

Authors

Perez, L. (deposition date: 2021-08-17, release date: 2021-10-13, Last modification date: 2024-10-23)

Primary citation

Fenwick, C.,Turelli, P.,Perez, L.,Pellaton, C.,Esteves-Leuenberger, L.,Farina, A.,Campos, J.,Lana, E.,Fiscalini, F.,Raclot, C.,Pojer, F.,Lau, K.,Demurtas, D.,Descatoire, M.,Joo, V.S.,Foglierini, M.,Noto, A.,Abdelnabi, R.,Foo, C.S.,Vangeel, L.,Neyts, J.,Du, W.,Bosch, B.J.,Veldman, G.,Leyssen, P.,Thiel, V.,LeGrand, R.,Levy, Y.,Trono, D.,Pantaleo, G.
A highly potent antibody effective against SARS-CoV-2 variants of concern.
Cell Rep, 37:109814-109814, 2021

PubMed Abstract: Control of the ongoing SARS-CoV-2 pandemic is endangered by the emergence of viral variants with increased transmission efficiency, resistance to marketed therapeutic antibodies, and reduced sensitivity to vaccine-induced immunity. Here, we screen B cells from COVID-19 donors and identify P5C3, a highly potent and broadly neutralizing monoclonal antibody with picomolar neutralizing activity against all SARS-CoV-2 variants of concern (VOCs) identified to date. Structural characterization of P5C3 Fab in complex with the spike demonstrates a neutralizing activity defined by a large buried surface area, highly overlapping with the receptor-binding domain (RBD) surface necessary for ACE2 interaction. We further demonstrate that P5C3 shows complete prophylactic protection in the SARS-CoV-2-infected hamster challenge model. These results indicate that P5C3 opens exciting perspectives either as a prophylactic agent in immunocompromised individuals with poor response to vaccination or as combination therapy in SARS-CoV-2-infected individuals.

PubMed: 34599871
DOI: 10.1016/j.celrep.2021.109814

Experimental method

ELECTRON MICROSCOPY (4.3 Å)