7PH7
Nanodisc reconstituted MsbA in complex with nanobodies, spin-labeled at position T68C
Summary for 7PH7
Entry DOI | 10.2210/pdb7ph7/pdb |
EMDB information | 13409 |
Descriptor | ATP-binding transport protein multicopy suppressor of htrB, Nb_MsbA#1, (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-4-[(3~{R})-3-nonanoyloxytetradecanoyl]oxy-5-[[(3~{R})-3-octanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{S},5~{S},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanylnonanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-oxan-2-yl]methoxy]-5-oxidanyl-oxane-2-carboxylic acid, ... (5 entities in total) |
Functional Keywords | abc transporter, nanobody, gd-dota, lipid a, membrane protein |
Biological source | Escherichia coli More |
Total number of polymer chains | 4 |
Total formula weight | 159771.46 |
Authors | Parey, K.,Januliene, D.,Galazzo, L.,Meier, G.,Vecchis, D.,Striednig, B.,Hilbi, H.,Schaefer, L.V.,Kuprov, I.,Bordignon, E.,Seeger, M.A.,Moeller, A. (deposition date: 2021-08-16, release date: 2022-08-24, Last modification date: 2022-10-26) |
Primary citation | Galazzo, L.,Meier, G.,Januliene, D.,Parey, K.,De Vecchis, D.,Striednig, B.,Hilbi, H.,Schafer, L.V.,Kuprov, I.,Moeller, A.,Bordignon, E.,Seeger, M.A. The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells. Sci Adv, 8:eabn6845-eabn6845, 2022 Cited by PubMed: 36223470DOI: 10.1126/sciadv.abn6845 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (4.1 Å) |
Structure validation
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