7PEL

CryoEM structure of simian T-cell lymphotropic virus intasome in complex with PP2A regulatory subunit B56 gamma

「6Z2Y」から置き換えられました

7PEL の概要

• エントリーDOI: 10.2210/pdb7pel/pdb
• 関連するPDBエントリー: 6QBT 6QBV 6QBW 6TJU 6TOQ
• EMDBエントリー: 11052
• 分子名称: Pol protein, Isoform 3 of PC4 and SFRS1-interacting protein,Isoform Gamma-2 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform, DNA (5'-D(*AP*CP*TP*GP*TP*GP*TP*TP*TP*GP*GP*CP*GP*CP*TP*TP*CP*TP*CP*TP*C)-3'), ... (5 entities in total)
• 機能のキーワード: integrase, transferase, deltaretrovirus, pp2a, b56, phosphatase, molecular mimicry, host factor, dna-binding, viral protein
• 由来する生物種: Simian T-lymphotropic virus 1; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 332223.95

構造登録者

Barski, M.,Pye, V.E.,Nans, A.,Cherepanov, P.,Maertens, G.N. (登録日: 2021-08-10, 公開日: 2021-08-25, 最終更新日: 2024-07-10)

主引用文献

Barski, M.S.,Minnell, J.J.,Hodakova, Z.,Pye, V.E.,Nans, A.,Cherepanov, P.,Maertens, G.N.
Cryo-EM structure of the deltaretroviral intasome in complex with the PP2A regulatory subunit B56gamma.
Nat Commun, 11:5043-, 2020

PubMed Abstract: Human T-cell lymphotropic virus type 1 (HTLV-1) is a deltaretrovirus and the most oncogenic pathogen. Many of the ~20 million HTLV-1 infected people will develop severe leukaemia or an ALS-like motor disease, unless a therapy becomes available. A key step in the establishment of infection is the integration of viral genetic material into the host genome, catalysed by the retroviral integrase (IN) enzyme. Here, we use X-ray crystallography and single-particle cryo-electron microscopy to determine the structure of the functional deltaretroviral IN assembled on viral DNA ends and bound to the B56γ subunit of its human host factor, protein phosphatase 2 A. The structure reveals a tetrameric IN assembly bound to two molecules of the phosphatase via a conserved short linear motif. Insight into the deltaretroviral intasome and its interaction with the host will be crucial for understanding the pattern of integration events in infected individuals and therefore bears important clinical implications.

PubMed: 33028863
DOI: 10.1038/s41467-020-18874-y

実験手法

ELECTRON MICROSCOPY (3.34 Å)