7PCX

X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE

7PCX の概要

• エントリーDOI: 10.2210/pdb7pcx/pdb
• 関連するPDBエントリー: 6ZVU 6ZVV 6ZVW 6ZVX 6ZVY 7PCW
• 分子名称: Haloalkane dehalogenase, [9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: haloalkane dehalogenase, halo, tag, halotag7, self-labeling protein, fluorophore, tetramethylrhodamine, hydrolase
• 由来する生物種: Rhodococcus sp.
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 204384.86

構造登録者

Tarnawski, M.,Frei, M.,Hiblot, J.,Johnsson, K. (登録日: 2021-08-04, 公開日: 2021-12-15, 最終更新日: 2024-11-13)

主引用文献

Frei, M.S.,Tarnawski, M.,Roberti, M.J.,Koch, B.,Hiblot, J.,Johnsson, K.
Engineered HaloTag variants for fluorescence lifetime multiplexing.
Nat.Methods, 19:65-70, 2022

PubMed Abstract: Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled with rhodamines. Combining these HaloTag variants enabled live-cell fluorescence lifetime multiplexing of three cellular targets in one spectral channel using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. Additionally, the brightest HaloTag variant showed up to 40% higher brightness in live-cell imaging applications.

PubMed: 34916672
DOI: 10.1038/s41592-021-01341-x

実験手法

X-RAY DIFFRACTION (1.4 Å)