7PCW
X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-M175W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE
Summary for 7PCW
Entry DOI | 10.2210/pdb7pcw/pdb |
Related | 6ZVU 6ZVV 6ZVW 6ZVX 6ZVY 7PCX |
Descriptor | Haloalkane dehalogenase, [9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, CHLORIDE ION, ... (4 entities in total) |
Functional Keywords | haloalkane dehalogenase, halo, tag, halotag7, self-labeling protein, fluorophore, tetramethylrhodamine, hydrolase |
Biological source | Rhodococcus sp. |
Total number of polymer chains | 4 |
Total formula weight | 135814.54 |
Authors | Tarnawski, M.,Frei, M.,Hiblot, J.,Johnsson, K. (deposition date: 2021-08-04, release date: 2021-12-15, Last modification date: 2024-01-31) |
Primary citation | Frei, M.S.,Tarnawski, M.,Roberti, M.J.,Koch, B.,Hiblot, J.,Johnsson, K. Engineered HaloTag variants for fluorescence lifetime multiplexing. Nat.Methods, 19:65-70, 2022 Cited by PubMed: 34916672DOI: 10.1038/s41592-021-01341-x PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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