6ZVY

X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165H-P174R LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE

Summary for 6ZVY

Entry DOI	10.2210/pdb6zvy/pdb
Related	6ZVU 6ZVV 6ZVW 6ZVX
Descriptor	Haloalkane dehalogenase, [9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, CHLORIDE ION, ... (5 entities in total)
Functional Keywords	haloalkane dehalogenase, halo, tag, halotag7, self-labeling protein, fluorophore, tetramethylrhodamine, hydrolase
Biological source	Rhodococcus sp.
Total number of polymer chains	2
Total formula weight	68305.81
Authors	Tarnawski, M.,Frei, M.,Hiblot, J.,Johnsson, K. (deposition date: 2020-07-27, release date: 2021-08-04, Last modification date: 2024-01-31)
Primary citation	Frei, M.S.,Tarnawski, M.,Roberti, M.J.,Koch, B.,Hiblot, J.,Johnsson, K. Engineered HaloTag variants for fluorescence lifetime multiplexing. Nat.Methods, 19:65-70, 2022 Cited by PubMed: 34916672 DOI: 10.1038/s41592-021-01341-x PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.4 Å)

Structure validation