7PBH
Structural characterization of Thorarchaeota profilin indicates a eukaryotic-like fold but with an extended N-terminus
Summary for 7PBH
| Entry DOI | 10.2210/pdb7pbh/pdb |
| NMR Information | BMRB: 50691 |
| Descriptor | Profilin (1 entity in total) |
| Functional Keywords | thor archaea profilin, protein binding |
| Biological source | Candidatus Thorarchaeota |
| Total number of polymer chains | 1 |
| Total formula weight | 15783.57 |
| Authors | Celestine, C. (deposition date: 2021-08-02, release date: 2022-08-10, Last modification date: 2024-02-21) |
| Primary citation | Inturi, R.,Lara, S.,Derweesh, M.,Chi, C.N. Structural Characterization of a Thorarchaeota Profilin Indicates Eukaryotic-Like Features but with an Extended N-Terminus. Adv Biol (Weinh), 6:e2101323-e2101323, 2022 Cited by PubMed Abstract: The emergence of the first eukaryotic cell is preceded by evolutionary events, which are still highly debatable. Clues of the exact sequence of events are beginning to emerge. Recent metagenomics analyses has uncovered the Asgard super-phylum as the closest yet known archaea host of eukaryotes. Some of these have been tested and confirmed experimentally. However, the bulk of eukaryotic signature proteins predicted to be encoded by the Asgard super-phylum have not been studied, and their true functions, at least in the context of a eukaryotic cell, are still elusive. For example, there are several different variants of the profilin within each Asgardian Achaea, and there are some conflicting results of their actual roles. Here, the 3D structure of profilin from Thorarchaeota is determined by nuclear magnetic resonance spectroscopy and shows that this profilin has a eukaryotic-like profilin with a rigid core and an extended N-terminus previously implicated in polyproline binding. In addition, it is also shown that Thorarchaeota Profilin co-localizes with eukaryotic actin in cultured HeLa cells. This finding reaffirms the notion that Asgardian encoded proteins possess eukaryotic-like characteristics and strengthen the likely existence of a complex cytoskeleton already in a last eukaryotic common ancestor. PubMed: 35429148DOI: 10.1002/adbi.202101323 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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