7P76

Re-engineered 2-deoxy-D-ribose-5-phosphate aldolase catalysing asymmetric Michael addition reactions, Schiff base complex with cinnamaldehyde

Summary for 7P76

• Entry DOI: 10.2210/pdb7p76/pdb
• Related: 7P75
• Descriptor: Deoxyribose-phosphate aldolase, (2E)-3-phenylprop-2-enal, GLYCEROL, ... (4 entities in total)
• Functional Keywords: protein engineering, directed evolution, aldolase, carboligase, michael addition, lyase, schiff base, cinnamaldehyde, carbon-carbon lyase
• Biological source: Escherichia coli 909945-2
• Total number of polymer chains: 12
• Total formula weight: 345882.86

Authors

Thunnissen, A.M.W.H.,Rozeboom, H.J.,Kunzendorf, A.,Poelarends, G.J. (deposition date: 2021-07-19, release date: 2021-10-27, Last modification date: 2024-01-31)

• Primary citation: Kunzendorf, A.,Xu, G.,van der Velde, J.J.H.,Rozeboom, H.J.,Thunnissen, A.W.H.,Poelarends, G.J.; Unlocking Asymmetric Michael Additions in an Archetypical Class I Aldolase by Directed Evolution.; Acs Catalysis, 11:13236-13243, 2021; PubMed: 34765282; DOI: 10.1021/acscatal.1c03911

Experimental method

X-RAY DIFFRACTION (1.9 Å)