7P5X

Mycobacterial RNAP with transcriptional activator PafBC

これはPDB形式変換不可エントリーです。

7P5X の概要

• エントリーDOI: 10.2210/pdb7p5x/pdb
• EMDBエントリー: 13205
• 分子名称: DNA-directed RNA polymerase subunit alpha, Transcriptional regulator-like protein, ZINC ION, ... (11 entities in total)
• 機能のキーワード: transcription, activator, sigma adaption, rna polymerase, rnap, pafb, pafc, pafbc, mycobacterium, smegmatis
• 由来する生物種: Mycolicibacterium smegmatis MC2 155; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 545245.01

構造登録者

Mueller, A.U.,Kummer, E.,Schilling, C.M.,Ban, N.,Weber-Ban, E. (登録日: 2021-07-15, 公開日: 2021-12-22, 最終更新日: 2024-07-17)

主引用文献

Muller, A.U.,Kummer, E.,Schilling, C.M.,Ban, N.,Weber-Ban, E.
Transcriptional control of mycobacterial DNA damage response by sigma adaptation.
Sci Adv, 7:eabl4064-eabl4064, 2021

PubMed Abstract: Transcriptional activator PafBC is the key regulator of the mycobacterial DNA damage response and controls around 150 genes, including genes involved in the canonical SOS response, through an unknown molecular mechanism. Using a combination of biochemistry and cryo–electron microscopy, we demonstrate that PafBC in the presence of single-stranded DNA activates transcription by reprogramming the canonical −10 and −35 promoter specificity of RNA polymerase associated with the housekeeping sigma subunit. We determine the structure of this transcription initiation complex, revealing a unique mode of promoter recognition, which we term “sigma adaptation.” PafBC inserts between DNA and sigma factor to mediate recognition of hybrid promoters lacking the −35 but featuring the canonical −10 and a PafBC-specific −26 element. Sigma adaptation may constitute a more general mechanism of transcriptional control in mycobacteria.

PubMed: 34851662
DOI: 10.1126/sciadv.abl4064

実験手法

ELECTRON MICROSCOPY (3.2 Å)