7OVC

Structure of the human UFC1 protein in complex with the UBA5 C-terminal UFC1-binding motif.

Summary for 7OVC

• Entry DOI: 10.2210/pdb7ovc/pdb
• NMR Information: BMRB: 34638
• Descriptor: Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5 (2 entities in total)
• Functional Keywords: ufm1, uba5, ufc1, ufmylation, complex structure, ligase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 22520.85

Authors

Wesch, W.,Loehr, F.,Rogova, N.,Doetsch, V.,Rogov, V.V. (deposition date: 2021-06-14, release date: 2021-08-04, Last modification date: 2024-06-19)

Primary citation

Wesch, N.,Lohr, F.,Rogova, N.,Dotsch, V.,Rogov, V.V.
A Concerted Action of UBA5 C-Terminal Unstructured Regions Is Important for Transfer of Activated UFM1 to UFC1.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: Ubiquitin fold modifier 1 (UFM1) is a member of the ubiquitin-like protein family. UFM1 undergoes a cascade of enzymatic reactions including activation by UBA5 (E1), transfer to UFC1 (E2) and selective conjugation to a number of target proteins via UFL1 (E3) enzymes. Despite the importance of ufmylation in a variety of cellular processes and its role in the pathogenicity of many human diseases, the molecular mechanisms of the ufmylation cascade remains unclear. In this study we focused on the biophysical and biochemical characterization of the interaction between UBA5 and UFC1. We explored the hypothesis that the unstructured C-terminal region of UBA5 serves as a regulatory region, controlling cellular localization of the elements of the ufmylation cascade and effective interaction between them. We found that the last 20 residues in UBA5 are pivotal for binding to UFC1 and can accelerate the transfer of UFM1 to UFC1. We solved the structure of a complex of UFC1 and a peptide spanning the last 20 residues of UBA5 by NMR spectroscopy. This structure in combination with additional NMR titration and isothermal titration calorimetry experiments revealed the mechanism of interaction and confirmed the importance of the C-terminal unstructured region in UBA5 for the ufmylation cascade.

PubMed: 34299007
DOI: 10.3390/ijms22147390

Experimental method

SOLUTION NMR