7OSO

The crystal structure of Erwinia tasmaniensis levansucrase in complex with (S)-1,2,4-butanentriol

Summary for 7OSO

• Entry DOI: 10.2210/pdb7oso/pdb
• Descriptor: Levansucrase (Beta-D-fructofuranosyl transferase), ZINC ION, (2~{S})-butane-1,2,4-triol, ... (4 entities in total)
• Functional Keywords: fructosyltransferase, levan, fos, transfructosylation, fructo-oligosaccharides, oligofructans, transferase
• Biological source: Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
• Total number of polymer chains: 1
• Total formula weight: 46441.58

Authors

Polsinelli, I.,Salomone-Stagni, M.,Benini, S. (deposition date: 2021-06-09, release date: 2022-06-22, Last modification date: 2024-02-07)

Primary citation

Polsinelli, I.,Salomone-Stagni, M.,Benini, S.
Erwinia tasmaniensis levansucrase shows enantiomer selection for (S)-1,2,4-butanetriol.
Acta Crystallogr.,Sect.F, 78:289-296, 2022

PubMed Abstract: Levansucrases are biotechnologically interesting fructosyltransferases due to their potential use in the enzymatic or chemo-enzymatic synthesis of glycosides of non-natural substrates relevant to pharmaceutical applications. The structure of Erwinia tasmaniensis levansucrase in complex with (S)-1,2,4-butanetriol and its biochemical characterization suggests the possible application of short aliphatic moieties containing polyols with defined stereocentres in fructosylation biotechnology. The structural information revealed that (S)-1,2,4-butanetriol mimics the natural substrate. The preference of the protein towards a specific 1,2,4-butanetriol enantiomer was assessed using microscale thermophoresis binding assays. Furthermore, the results obtained and the structural comparison of levansucrases and inulosucrases suggest that the fructose binding modes could differ in fructosyltransferases from Gram-positive and Gram-negative bacteria.

PubMed: 35924596
DOI: 10.1107/S2053230X2200680X

Experimental method

X-RAY DIFFRACTION (1.4 Å)