7OSN
IRED361 from Micromonospora sp. in complex with NADP+
Summary for 7OSN
| Entry DOI | 10.2210/pdb7osn/pdb |
| Descriptor | 6-phosphogluconate dehydrogenase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total) |
| Functional Keywords | amine, nadp, imine, oxidoreductase |
| Biological source | Micromonospora sp. Rc5 |
| Total number of polymer chains | 6 |
| Total formula weight | 193848.74 |
| Authors | Gilio, A.K.,Harawa, V.,Turner, N.,Grogan, G.J. (deposition date: 2021-06-09, release date: 2022-06-22, Last modification date: 2024-01-31) |
| Primary citation | Harawa, V.,Thorpe, T.W.,Marshall, J.R.,Sangster, J.J.,Gilio, A.K.,Pirvu, L.,Heath, R.S.,Angelastro, A.,Finnigan, J.D.,Charnock, S.J.,Nafie, J.W.,Grogan, G.,Whitehead, R.C.,Turner, N.J. Synthesis of Stereoenriched Piperidines via Chemo-Enzymatic Dearomatization of Activated Pyridines. J.Am.Chem.Soc., 144:21088-21095, 2022 Cited by PubMed Abstract: The development of efficient and sustainable methods for the synthesis of nitrogen heterocycles is an important goal for the chemical industry. In particular, substituted chiral piperidines are prominent targets due to their prevalence in medicinally relevant compounds and their precursors. A potential biocatalytic approach to the synthesis of this privileged scaffold would be the asymmetric dearomatization of readily assembled activated pyridines. However, nature is yet to yield a suitable biocatalyst specifically for this reaction. Here, by combining chemical synthesis and biocatalysis, we present a general chemo-enzymatic approach for the asymmetric dearomatization of activated pyridines for the preparation of substituted piperidines with precise stereochemistry. The key step involves a stereoselective one-pot amine oxidase/ene imine reductase cascade to convert N-substituted tetrahydropyridines to stereo-defined 3- and 3,4-substituted piperidines. This chemo-enzymatic approach has proved useful for key transformations in the syntheses of antipsychotic drugs Preclamol and OSU-6162, as well as for the preparation of two important intermediates in synthetic routes of the ovarian cancer monotherapeutic Niraparib. PubMed: 36350999DOI: 10.1021/jacs.2c07143 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.55 Å) |
Structure validation
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