7OKO
Structure of the outer-membrane core complex (outer ring) from a conjugative type IV secretion system
This is a non-PDB format compatible entry.
Summary for 7OKO
| Entry DOI | 10.2210/pdb7oko/pdb |
| EMDB information | 12963 |
| Descriptor | Type IV conjugative transfer system lipoprotein TraV, TraB, Type-F conjugative transfer system secretin TraK (3 entities in total) |
| Functional Keywords | type iv secretion system, f plasmid, outer-membrane core complex, conjugation, membrane protein |
| Biological source | Salmonella enterica (Salmonella choleraesuis) More |
| Total number of polymer chains | 65 |
| Total formula weight | 1164938.25 |
| Authors | Amin, H.,Ilangovan, A.,Costa, T.R.D. (deposition date: 2021-05-18, release date: 2021-12-08, Last modification date: 2024-07-10) |
| Primary citation | Amin, H.,Ilangovan, A.,Costa, T.R.D. Architecture of the outer-membrane core complex from a conjugative type IV secretion system. Nat Commun, 12:6834-6834, 2021 Cited by PubMed Abstract: Conjugation is one of the most important processes that bacteria utilize to spread antibiotic resistance genes among bacterial populations. Interbacterial DNA transfer requires a large double membrane-spanning nanomachine called the type 4 secretion system (T4SS) made up of the inner-membrane complex (IMC), the outer-membrane core complex (OMCC) and the conjugative pilus. The iconic F plasmid-encoded T4SS has been central in understanding conjugation for several decades, however atomic details of its structure are not known. Here, we report the structure of a complete conjugative OMCC encoded by the pED208 plasmid from E. coli, solved by cryo-electron microscopy at 3.3 Å resolution. This 2.1 MDa complex has a unique arrangement with two radial concentric rings, each having a different symmetry eventually contributing to remarkable differences in protein stoichiometry and flexibility in comparison to other OMCCs. Our structure suggests that F-OMCC is a highly dynamic complex, with implications for pilus extension and retraction during conjugation. PubMed: 34824240DOI: 10.1038/s41467-021-27178-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
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