7OI0

E.coli delta rbfA pre-30S ribosomal subunit class D

Summary for 7OI0

• Entry DOI: 10.2210/pdb7oi0/pdb
• EMDB information: 12916
• Descriptor: 30S ribosomal protein S4, 30S ribosomal protein S20, 16S rRNA, ... (12 entities in total)
• Functional Keywords: ribosome, 30s subunit, rna, rbfa
• Biological source: Escherichia coli BW25113; More
• Total number of polymer chains: 11
• Total formula weight: 627764.94

Authors

Maksimova, E.,Korepanov, A.,Baymukhametov, T.,Kravchenko, O.,Stolboushkina, E. (deposition date: 2021-05-11, release date: 2021-07-14, Last modification date: 2024-07-10)

Primary citation

Maksimova, E.M.,Korepanov, A.P.,Kravchenko, O.V.,Baymukhametov, T.N.,Myasnikov, A.G.,Vassilenko, K.S.,Afonina, Z.A.,Stolboushkina, E.A.
RbfA Is Involved in Two Important Stages of 30S Subunit Assembly: Formation of the Central Pseudoknot and Docking of Helix 44 to the Decoding Center.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: Ribosome biogenesis is a highly coordinated and complex process that requires numerous assembly factors that ensure prompt and flawless maturation of ribosomal subunits. Despite the increasing amount of data collected, the exact role of most assembly factors and mechanistic details of their operation remain unclear, mainly due to the shortage of high-resolution structural information. Here, using cryo-electron microscopy, we characterized 30S ribosomal particles isolated from an   strain with a deleted gene for the RbfA factor. The cryo-EM maps for pre-30S subunits were divided into six classes corresponding to consecutive assembly intermediates: from the particles with a completely unresolved head domain and unfolded central pseudoknot to almost mature 30S subunits with well-resolved body, platform, and head domains and partially distorted helix 44. The structures of two predominant 30S intermediates belonging to most populated classes obtained at 2.7 Å resolutions indicate that RbfA acts at two distinctive 30S assembly stages: early formation of the central pseudoknot including folding of the head, and positioning of helix 44 in the decoding center at a later stage. Additionally, it was shown that the formation of the central pseudoknot may promote stabilization of the head domain, likely through the RbfA-dependent maturation of the neck helix 28. An update to the model of factor-dependent 30S maturation is proposed, suggesting that RfbA is involved in most of the subunit assembly process.

PubMed: 34200244
DOI: 10.3390/ijms22116140

Experimental method

ELECTRON MICROSCOPY (2.76 Å)