7OHF

Cryo-EM structure of pyrococcus furiosus apoferritin in nanofluidic channels

Summary for 7OHF

• Entry DOI: 10.2210/pdb7ohf/pdb
• EMDB information: 12901
• Descriptor: Ferritin (1 entity in total)
• Functional Keywords: iron storage, metal transport
• Biological source: Pyrococcus furiosus COM1
• Total number of polymer chains: 1
• Total formula weight: 20535.40

Authors

Huber, S.T.,Sarajlic, E.,Huijink, R.,Evers, W.H.,Jakobi, A.J. (deposition date: 2021-05-10, release date: 2021-08-11, Last modification date: 2024-07-10)

Primary citation

Huber, S.T.,Sarajlic, E.,Huijink, R.,Weis, F.,Evers, W.H.,Jakobi, A.J.
Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes.
Elife, 11:-, 2022

PubMed Abstract: Cryogenic electron microscopy has become an essential tool for structure determination of biological macromolecules. In practice, the difficulty to reliably prepare samples with uniform ice thickness still represents a barrier for routine high-resolution imaging and limits the current throughput of the technique. We show that a nanofluidic sample support with well-defined geometry can be used to prepare cryo-EM specimens with reproducible ice thickness from picoliter sample volumes. The sample solution is contained in electron-transparent nanochannels that provide uniform thickness gradients without further optimisation and eliminate the potentially destructive air-water interface. We demonstrate the possibility to perform high-resolution structure determination with three standard protein specimens. Nanofabricated sample supports bear potential to automate the cryo-EM workflow, and to explore new frontiers for cryo-EM applications such as time-resolved imaging and high-throughput screening.

PubMed: 35060902
DOI: 10.7554/eLife.72629

Experimental method

ELECTRON MICROSCOPY (3 Å)