7OFQ

The archaellum of Methanocaldococcus villosus

7OFQ の概要

• エントリーDOI: 10.2210/pdb7ofq/pdb
• EMDBエントリー: 12875
• 分子名称: Archaellin, CALCIUM ION, UNKNOWN LIGAND, ... (4 entities in total)
• 機能のキーワード: archaellum, archaellin, methanocaldococcus villosus, cryoem, helical reconstruction, protein fibril
• 由来する生物種: Methanocaldococcus villosus; 詳細
• タンパク質・核酸の鎖数: 45
• 化学式量合計: 999762.07

構造登録者

Isupov, M.,Gambelli, L.,Daum, B. (登録日: 2021-05-05, 公開日: 2022-01-19, 最終更新日: 2025-10-01)

主引用文献

Gambelli, L.,Isupov, M.N.,Conners, R.,McLaren, M.,Bellack, A.,Gold, V.,Rachel, R.,Daum, B.
An archaellum filament composed of two alternating subunits.
Nat Commun, 13:710-710, 2022

PubMed Abstract: Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in the same operon; however, previous structural studies indicated that archaellum filaments mainly consist of only one protein species. Here, we use electron cryo-microscopy to elucidate the structure of the archaellum from Methanocaldococcus villosus at 3.08 Å resolution. The filament is composed of two alternating archaellins, suggesting that the architecture and assembly of archaella is more complex than previously thought. Moreover, we identify structural elements that may contribute to the filament's flexibility.

PubMed: 35132062
DOI: 10.1038/s41467-022-28337-1

実験手法

ELECTRON MICROSCOPY (3.08 Å)