7OCD

Resting state GluA1/A2 heterotetramer in complex with auxiliary subunit TARP gamma 8 (LBD-TMD)

7OCD の概要

• エントリーDOI: 10.2210/pdb7ocd/pdb
• EMDBエントリー: 12804
• 分子名称: Isoform Flip of Glutamate receptor 1, Glutamate receptor 2, Voltage-dependent calcium channel gamma-8 subunit, ... (5 entities in total)
• 機能のキーワード: ampar, ion channels, neurotransmission, membrane protein
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 491055.97

構造登録者

Zhang, D.,Watson, J.F.,Matthews, P.M.,Cais, O.,Greger, I.H. (登録日: 2021-04-26, 公開日: 2021-06-09, 最終更新日: 2024-10-23)

主引用文献

Zhang, D.,Watson, J.F.,Matthews, P.M.,Cais, O.,Greger, I.H.
Gating and modulation of a hetero-octameric AMPA glutamate receptor.
Nature, 594:454-458, 2021

PubMed Abstract: AMPA receptors (AMPARs) mediate the majority of excitatory transmission in the brain and enable the synaptic plasticity that underlies learning. A diverse array of AMPAR signalling complexes are established by receptor auxiliary subunits, which associate with the AMPAR in various combinations to modulate trafficking, gating and synaptic strength. However, their mechanisms of action are poorly understood. Here we determine cryo-electron microscopy structures of the heteromeric GluA1-GluA2 receptor assembled with both TARP-γ8 and CNIH2, the predominant AMPAR complex in the forebrain, in both resting and active states. Two TARP-γ8 and two CNIH2 subunits insert at distinct sites beneath the ligand-binding domains of the receptor, with site-specific lipids shaping each interaction and affecting the gating regulation of the AMPARs. Activation of the receptor leads to asymmetry between GluA1 and GluA2 along the ion conduction path and an outward expansion of the channel triggers counter-rotations of both auxiliary subunit pairs, promoting the active-state conformation. In addition, both TARP-γ8 and CNIH2 pivot towards the pore exit upon activation, extending their reach for cytoplasmic receptor elements. CNIH2 achieves this through its uniquely extended M2 helix, which has transformed this endoplasmic reticulum-export factor into a powerful AMPAR modulator that is capable of providing hippocampal pyramidal neurons with their integrative synaptic properties.

PubMed: 34079129
DOI: 10.1038/s41586-021-03613-0

実験手法

ELECTRON MICROSCOPY (3.5 Å)