7OBQ

SRP-SR at the distal site conformation

7OBQ の概要

• エントリーDOI: 10.2210/pdb7obq/pdb
• EMDBエントリー: 12799
• 分子名称: SRP RNA, GUANOSINE-5'-TRIPHOSPHATE, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, ... (11 entities in total)
• 機能のキーワード: srp srp receptor, co-translational protein targeting, endoplasmic reticulum, signal peptide, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 400879.42

構造登録者

Jomaa, A.,Ban, N. (登録日: 2021-04-23, 公開日: 2021-07-21, 最終更新日: 2024-07-10)

主引用文献

Jomaa, A.,Eitzinger, S.,Zhu, Z.,Chandrasekar, S.,Kobayashi, K.,Shan, S.O.,Ban, N.
Molecular mechanism of cargo recognition and handover by the mammalian signal recognition particle.
Cell Rep, 36:109350-109350, 2021

PubMed Abstract: Co-translational protein targeting to membranes by the signal recognition particle (SRP) is a universally conserved pathway from bacteria to humans. In mammals, SRP and its receptor (SR) have many additional RNA features and protein components compared to the bacterial system, which were recently shown to play regulatory roles. Due to its complexity, the mammalian SRP targeting process is mechanistically not well understood. In particular, it is not clear how SRP recognizes translating ribosomes with exposed signal sequences and how the GTPase activity of SRP and SR is regulated. Here, we present electron cryo-microscopy structures of SRP and SRP·SR in complex with the translating ribosome. The structures reveal the specific molecular interactions between SRP and the emerging signal sequence and the elements that regulate GTPase activity of SRP·SR. Our results suggest the molecular mechanism of how eukaryote-specific elements regulate the early and late stages of SRP-dependent protein targeting.

PubMed: 34260909
DOI: 10.1016/j.celrep.2021.109350

実験手法

ELECTRON MICROSCOPY (3.9 Å)