7OB4

Cryo-EM structure of a twisted-dimer transthyretin amyloid fibril from vitreous body of the eye

Summary for 7OB4

• Entry DOI: 10.2210/pdb7ob4/pdb
• EMDB information: 12794
• Descriptor: Transthyretin (1 entity in total)
• Functional Keywords: amyloid fibril, transthyretin, misfolding, protein fibril, attr amyloidosis, v30m variant, ex vivo, vitreous body
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 14
• Total formula weight: 193331.96

Authors

Iakovleva, I.,Sauer-Eriksson, A.E. (deposition date: 2021-04-21, release date: 2021-12-15, Last modification date: 2024-07-10)

Primary citation

Iakovleva, I.,Hall, M.,Oelker, M.,Sandblad, L.,Anan, I.,Sauer-Eriksson, A.E.
Structural basis for transthyretin amyloid formation in vitreous body of the eye.
Nat Commun, 12:7141-7141, 2021

PubMed Abstract: Amyloid transthyretin (ATTR) amyloidosis is characterized by the abnormal accumulation of ATTR fibrils in multiple organs. However, the structure of ATTR fibrils from the eye is poorly understood. Here, we used cryo-EM to structurally characterize vitreous body ATTR fibrils. These structures were distinct from previously characterized heart fibrils, even though both have the same mutation and type A pathology. Differences were observed at several structural levels: in both the number and arrangement of protofilaments, and the conformation of the protein fibril in each layer of protofilaments. Thus, our results show that ATTR protein structure and its assembly into protofilaments in the type A fibrils can vary between patients carrying the same mutation. By analyzing and matching the interfaces between the amino acids in the ATTR fibril with those in the natively folded TTR, we are able to propose a mechanism for the structural conversion of TTR into a fibrillar form.

PubMed: 34880242
DOI: 10.1038/s41467-021-27481-4

Experimental method

ELECTRON MICROSCOPY (3.22 Å)