7OB0
Structure of RsLOV d2 variant
Summary for 7OB0
| Entry DOI | 10.2210/pdb7ob0/pdb |
| Related | 4HIA 7OBZ |
| Descriptor | LOV protein, CALCIUM ION, FLAVIN MONONUCLEOTIDE, ... (7 entities in total) |
| Functional Keywords | photoreceptor, light-oxygen-voltage, flavin, per-arnt-sim, signaling protein |
| Biological source | Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) |
| Total number of polymer chains | 4 |
| Total formula weight | 80434.34 |
| Authors | Moeglich, A.,Krafft, T.G.A.,Weyand, M. (deposition date: 2021-04-20, release date: 2021-06-30, Last modification date: 2024-01-31) |
| Primary citation | Dietler, J.,Schubert, R.,Krafft, T.G.A.,Meiler, S.,Kainrath, S.,Richter, F.,Schweimer, K.,Weyand, M.,Janovjak, H.,Moglich, A. A Light-Oxygen-Voltage Receptor Integrates Light and Temperature. J.Mol.Biol., 433:167107-167107, 2021 Cited by PubMed Abstract: Sensory photoreceptors enable organisms to adjust their physiology, behavior, and development in response to light, generally with spatiotemporal acuity and reversibility. These traits underlie the use of photoreceptors as genetically encoded actuators to alter by light the state and properties of heterologous organisms. Subsumed as optogenetics, pertinent approaches enable regulating diverse cellular processes, not least gene expression. Here, we controlled the widely used Tet repressor by coupling to light-oxygen-voltage (LOV) modules that either homodimerize or dissociate under blue light. Repression could thus be elevated or relieved, and consequently protein expression was modulated by light. Strikingly, the homodimeric RsLOV module from Rhodobacter sphaeroides not only dissociated under light but intrinsically reacted to temperature. The limited light responses of wild-type RsLOV at 37 °C were enhanced in two variants that exhibited closely similar photochemistry and structure. One variant improved the weak homodimerization affinity of 40 µM by two-fold and thus also bestowed light sensitivity on a receptor tyrosine kinase. Certain photoreceptors, exemplified by RsLOV, can evidently moonlight as temperature sensors which immediately bears on their application in optogenetics and biotechnology. Properly accounted for, the temperature sensitivity can be leveraged for the construction of signal-responsive cellular circuits. PubMed: 34146595DOI: 10.1016/j.jmb.2021.167107 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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