7OB0

Structure of RsLOV d2 variant

7OB0 の概要

• エントリーDOI: 10.2210/pdb7ob0/pdb
• 関連するPDBエントリー: 4HIA 7OBZ
• 分子名称: LOV protein, CALCIUM ION, FLAVIN MONONUCLEOTIDE, ... (7 entities in total)
• 機能のキーワード: photoreceptor, light-oxygen-voltage, flavin, per-arnt-sim, signaling protein
• 由来する生物種: Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 80434.34

構造登録者

Moeglich, A.,Krafft, T.G.A.,Weyand, M. (登録日: 2021-04-20, 公開日: 2021-06-30, 最終更新日: 2024-01-31)

主引用文献

Dietler, J.,Schubert, R.,Krafft, T.G.A.,Meiler, S.,Kainrath, S.,Richter, F.,Schweimer, K.,Weyand, M.,Janovjak, H.,Moglich, A.
A Light-Oxygen-Voltage Receptor Integrates Light and Temperature.
J.Mol.Biol., 433:167107-167107, 2021

PubMed Abstract: Sensory photoreceptors enable organisms to adjust their physiology, behavior, and development in response to light, generally with spatiotemporal acuity and reversibility. These traits underlie the use of photoreceptors as genetically encoded actuators to alter by light the state and properties of heterologous organisms. Subsumed as optogenetics, pertinent approaches enable regulating diverse cellular processes, not least gene expression. Here, we controlled the widely used Tet repressor by coupling to light-oxygen-voltage (LOV) modules that either homodimerize or dissociate under blue light. Repression could thus be elevated or relieved, and consequently protein expression was modulated by light. Strikingly, the homodimeric RsLOV module from Rhodobacter sphaeroides not only dissociated under light but intrinsically reacted to temperature. The limited light responses of wild-type RsLOV at 37 °C were enhanced in two variants that exhibited closely similar photochemistry and structure. One variant improved the weak homodimerization affinity of 40 µM by two-fold and thus also bestowed light sensitivity on a receptor tyrosine kinase. Certain photoreceptors, exemplified by RsLOV, can evidently moonlight as temperature sensors which immediately bears on their application in optogenetics and biotechnology. Properly accounted for, the temperature sensitivity can be leveraged for the construction of signal-responsive cellular circuits.

PubMed: 34146595
DOI: 10.1016/j.jmb.2021.167107

実験手法

X-RAY DIFFRACTION (1.9 Å)