7OA8

PilC minor pilin of Streptococcus sanguinis SK36 type IV pili

Summary for 7OA8

• Entry DOI: 10.2210/pdb7oa8/pdb
• Descriptor: PilC minor pilin (2 entities in total)
• Functional Keywords: pilin, structural protein
• Biological source: Streptococcus sanguinis
• Total number of polymer chains: 1
• Total formula weight: 51515.57

Authors

Sheppard, D.,Pelicic, V. (deposition date: 2021-04-19, release date: 2022-05-04, Last modification date: 2024-01-31)

Primary citation

Shahin, M.,Sheppard, D.,Raynaud, C.,Berry, J.L.,Gurung, I.,Silva, L.M.,Feizi, T.,Liu, Y.,Pelicic, V.
Characterization of a glycan-binding complex of minor pilins completes the analysis of Streptococcus sanguinis type 4 pili subunits.
Proc.Natl.Acad.Sci.USA, 120:e2216237120-e2216237120, 2023

PubMed Abstract: Type 4 filaments (T4F)-of which type 4 pili (T4P) are the archetype-are a superfamily of nanomachines nearly ubiquitous in prokaryotes. T4F are polymers of one major pilin, which also contain minor pilins whose roles are often poorly understood. Here, we complete the structure/function analysis of the full set of T4P pilins in the opportunistic bacterial pathogen . We determined the structure of the minor pilin PilA, which is unexpectedly similar to one of the subunits of a tip-located complex of four minor pilins, widely conserved in T4F. We found that PilA interacts and dramatically stabilizes the minor pilin PilC. We determined the structure of PilC, showing that it is a modular pilin with a lectin module binding a subset of glycans prevalent in the human glycome, the host of . Altogether, our findings support a model whereby the minor pilins in T4P form a tip-located complex promoting adhesion to various host receptors. This has general implications for T4F.

PubMed: 36626560
DOI: 10.1073/pnas.2216237120

Experimental method

X-RAY DIFFRACTION (1.6 Å)