7O3B

Crystal structure of the TTBK2-CEP164 complex bound to a camelid nanobody

Summary for 7O3B

• Entry DOI: 10.2210/pdb7o3b/pdb
• Related: 7NWJ 7O06 7O0S
• Descriptor: Nanobody 36Z, Tau-tubulin kinase 2,Centrosomal protein of 164 kDa (3 entities in total)
• Functional Keywords: centriole, centrosome, basal body, ciliogenesis, structural protein
• Biological source: Camelidae mixed library; More
• Total number of polymer chains: 6
• Total formula weight: 84450.78

Authors

e Silva, I.R.,van Breugel, M. (deposition date: 2021-04-01, release date: 2021-09-15, Last modification date: 2024-01-31)

Primary citation

Rosa E Silva, I.,Bino, L.,Johnson, C.M.,Rutherford, T.J.,Neuhaus, D.,Andreeva, A.,Cajanek, L.,van Breugel, M.
Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.
Structure, 30:114-128.e9, 2022

PubMed Abstract: Cilia formation is essential for human life. One of the earliest events in the ciliogenesis program is the recruitment of tau-tubulin kinase 2 (TTBK2) by the centriole distal appendage component CEP164. Due to the lack of high-resolution structural information on this complex, it is unclear how it is affected in human ciliopathies such as nephronophthisis. Furthermore, it is poorly understood if binding to CEP164 influences TTBK2 activities. Here, we present a detailed biochemical, structural, and functional analysis of the CEP164-TTBK2 complex and demonstrate how it is compromised by two ciliopathic mutations in CEP164. Moreover, we also provide insights into how binding to CEP164 is coordinated with TTBK2 activities. Together, our data deepen our understanding of a crucial step in cilia formation and will inform future studies aimed at restoring CEP164 functionality in a debilitating human ciliopathy.

PubMed: 34499853
DOI: 10.1016/j.str.2021.08.007

Experimental method

X-RAY DIFFRACTION (2.4 Å)