7O2L

Yeast 20S proteasome in complex with the covalently bound inhibitor b-lactone (2R,3S)-3-isopropyl-4-oxo-2-oxetane-carboxylate (IOC)

7O2L の概要

• エントリーDOI: 10.2210/pdb7o2l/pdb
• 関連するPDBエントリー: 3E47
• 分子名称: HLJ1_G0039880.mRNA.1.CDS.1, Proteasome subunit beta, Proteasome subunit beta type-5, ... (18 entities in total)
• 機能のキーワード: hydrolase-hydrolase inhibitor complex, proteasome, b-lactone, binding analysis, hydrolase
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 732277.12

構造登録者

Shi, Y.M.,Hirschmann, M.,Shi, Y.N.,Shabbir, A.,Abebew, D.,Tobias, N.J.,Gruen, P.,Crames, J.J.,Poeschel, L.,Kuttenlochner, W.,Richter, C.,Herrmann, J.,Mueller, R.,Thanwisai, A.,Pidot, S.J.,Stinear, T.P.,Groll, M.,Kim, Y.,Bode, H. (登録日: 2021-03-30, 公開日: 2022-04-13, 最終更新日: 2025-10-01)

主引用文献

Shi, Y.M.,Hirschmann, M.,Shi, Y.N.,Ahmed, S.,Abebew, D.,Tobias, N.J.,Grun, P.,Crames, J.J.,Poschel, L.,Kuttenlochner, W.,Richter, C.,Herrmann, J.,Muller, R.,Thanwisai, A.,Pidot, S.J.,Stinear, T.P.,Groll, M.,Kim, Y.,Bode, H.B.
Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria.
Nat.Chem., 14:701-712, 2022

PubMed Abstract: Microorganisms contribute to the biology and physiology of eukaryotic hosts and affect other organisms through natural products. Xenorhabdus and Photorhabdus (XP) living in mutualistic symbiosis with entomopathogenic nematodes generate natural products to mediate bacteria-nematode-insect interactions. However, a lack of systematic analysis of the XP biosynthetic gene clusters (BGCs) has limited the understanding of how natural products affect interactions between the organisms. Here we combine pangenome and sequence similarity networks to analyse BGCs from 45 XP strains that cover all sequenced strains in our collection and represent almost all XP taxonomy. The identified 1,000 BGCs belong to 176 families. The most conserved families are denoted by 11 BGC classes. We homologously (over)express the ubiquitous and unique BGCs and identify compounds featuring unusual architectures. The bioactivity evaluation demonstrates that the prevalent compounds are eukaryotic proteasome inhibitors, virulence factors against insects, metallophores and insect immunosuppressants. These findings explain the functional basis of bacterial natural products in this tripartite relationship.

PubMed: 35469007
DOI: 10.1038/s41557-022-00923-2

実験手法

X-RAY DIFFRACTION (3 Å)