7NUR
Structure of the Toxoplasma gondii kinase Ron13, kinase-dead mutant
Summary for 7NUR
| Entry DOI | 10.2210/pdb7nur/pdb |
| EMDB information | 12600 |
| Descriptor | Protein kinase domain-containing protein (1 entity in total) |
| Functional Keywords | secretory pathway, kinase, transferase |
| Biological source | Toxoplasma gondii (strain ATCC 50853 / GT1) |
| Total number of polymer chains | 1 |
| Total formula weight | 126419.68 |
| Authors | Korkhov, V.M.,Mehta, V. (deposition date: 2021-03-13, release date: 2021-05-26, Last modification date: 2024-10-16) |
| Primary citation | Lentini, G.,Ben Chaabene, R.,Vadas, O.,Ramakrishnan, C.,Mukherjee, B.,Mehta, V.,Lunghi, M.,Grossmann, J.,Maco, B.,Visentin, R.,Hehl, A.B.,Korkhov, V.M.,Soldati-Favre, D. Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion. Nat Commun, 12:3788-3788, 2021 Cited by PubMed Abstract: Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete their rhoptry content and insert a complex of RON proteins on the cytoplasmic side of the host cell membrane providing an anchor to which the parasite tethers. Here we show that a rhoptry-resident kinase RON13 is a key virulence factor that plays a crucial role in host cell entry. Cryo-EM, kinase assays, phosphoproteomics and cellular analyses reveal that RON13 is a secretory pathway kinase of atypical structure that phosphorylates rhoptry proteins including the components of the RON complex. Ultimately, RON13 kinase activity controls host cell invasion by anchoring the moving junction at the parasite-host cell interface. PubMed: 34145271DOI: 10.1038/s41467-021-24083-y PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.125 Å) |
Structure validation
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