7NS6
SARS-CoV-2 Spike (dimers) in complex with six Fu2 nanobodies
Summary for 7NS6
| Entry DOI | 10.2210/pdb7ns6/pdb |
| EMDB information | 12561 |
| Descriptor | Fu2 nanobody, Spike glycoprotein,Fibritin, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total) |
| Functional Keywords | nanobody, spike, complex, dimer, viral protein |
| Biological source | Vicugna pacos (alpaca) More |
| Total number of polymer chains | 12 |
| Total formula weight | 961368.94 |
| Authors | Das, H.,Hallberg, B.M. (deposition date: 2021-03-05, release date: 2022-02-02, Last modification date: 2024-10-23) |
| Primary citation | Hanke, L.,Das, H.,Sheward, D.J.,Perez Vidakovics, L.,Urgard, E.,Moliner-Morro, A.,Kim, C.,Karl, V.,Pankow, A.,Smith, N.L.,Porebski, B.,Fernandez-Capetillo, O.,Sezgin, E.,Pedersen, G.K.,Coquet, J.M.,Hallberg, B.M.,Murrell, B.,McInerney, G.M. A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo. Nat Commun, 13:155-155, 2022 Cited by PubMed Abstract: Antibodies binding to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike have therapeutic promise, but emerging variants show the potential for virus escape. This emphasizes the need for therapeutic molecules with distinct and novel neutralization mechanisms. Here we describe the isolation of a nanobody that interacts simultaneously with two RBDs from different spike trimers of SARS-CoV-2, rapidly inducing the formation of spike trimer-dimers leading to the loss of their ability to attach to the host cell receptor, ACE2. We show that this nanobody potently neutralizes SARS-CoV-2, including the beta and delta variants, and cross-neutralizes SARS-CoV. Furthermore, we demonstrate the therapeutic potential of the nanobody against SARS-CoV-2 and the beta variant in a human ACE2 transgenic mouse model. This naturally elicited bispecific monomeric nanobody establishes an uncommon strategy for potent inactivation of viral antigens and represents a promising antiviral against emerging SARS-CoV-2 variants. PubMed: 35013189DOI: 10.1038/s41467-021-27610-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.18 Å) |
Structure validation
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