7NS2
Virion of Leishmania RNA virus 1
Summary for 7NS2
| Entry DOI | 10.2210/pdb7ns2/pdb |
| Related | 6y83 |
| EMDB information | 10722 12558 |
| Descriptor | Capsid protein (1 entity in total) |
| Functional Keywords | leishmania guyanensis, native virion structure, mucocutaneous leishmaniasis, virus, totiviridae |
| Biological source | Leishmania RNA virus 1 - LgM5313 |
| Total number of polymer chains | 2 |
| Total formula weight | 172863.48 |
| Authors | Prochazkova, M.,Grybchuk, D.,Fuzik, T. (deposition date: 2021-03-05, release date: 2022-09-21, Last modification date: 2024-10-23) |
| Primary citation | Prochazkova, M.,Fuzik, T.,Grybchuk, D.,Yurchenko, V.,Plevka, P. Virion structure of Leishmania RNA virus 1. Virology, 577:149-154, 2022 Cited by PubMed Abstract: The presence of Leishmania RNA virus 1 (LRV1) enables Leishmania protozoan parasites to cause more severe disease than the virus-free strains. The structure of LRV1 virus-like particles has been determined previously, however, the structure of the LRV1 virion has not been characterized. Here we used cryo-electron microscopy and single-particle reconstruction to determine the structures of the LRV1 virion and empty particle isolated from Leishmania guyanensis to resolutions of 4.0 Å and 3.6 Å, respectively. The capsid of LRV1 is built from sixty dimers of capsid proteins organized with icosahedral symmetry. RNA genomes of totiviruses are replicated inside the virions by RNA polymerases expressed as C-terminal extensions of a sub-population of capsid proteins. Most of the virions probably contain one or two copies of the RNA polymerase, however, the location of the polymerase domains in LRV1 capsid could not be identified, indicating that it varies among particles. Importance. Every year over 200 000 people contract leishmaniasis and more than five hundred people die of the disease. The mucocutaneous form of leishmaniasis produces lesions that can destroy the mucous membranes of the nose, mouth, and throat. Leishmania parasites carrying Leishmania RNA virus 1 (LRV1) are predisposed to cause aggravated symptoms in the mucocutaneous form of leishmaniasis. Here, we present the structure of the LRV1 virion determined using cryo-electron microscopy. PubMed: 36371873DOI: 10.1016/j.virol.2022.09.014 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.63 Å) |
Structure validation
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