7NR3

Discovery of ASTX029, a clinical candidate which modulates the phosphorylation and catalytic activity of ERK1/2

Summary for 7NR3

• Entry DOI: 10.2210/pdb7nr3/pdb
• Related: 7NQQ 7NQW
• Descriptor: Mitogen-activated protein kinase 1, SULFATE ION, 6-[5-chloranyl-2-(oxan-4-ylamino)pyrimidin-4-yl]-2-[2-oxidanylidene-2-(1,2,4,5-tetrahydro-3-benzazepin-3-yl)ethyl]-3~{H}-isoindol-1-one, ... (4 entities in total)
• Functional Keywords: serine-threonine kinase, protein kinase, signal transduction, map kinase, signaling protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 43276.08

Authors

O'Reilly, M. (deposition date: 2021-03-02, release date: 2021-10-06, Last modification date: 2024-11-06)

Primary citation

Heightman, T.D.,Berdini, V.,Bevan, L.,Buck, I.M.,Carr, M.G.,Courtin, A.,Coyle, J.E.,Day, J.E.H.,East, C.,Fazal, L.,Griffiths-Jones, C.M.,Howard, S.,Kucia-Tran, J.,Martins, V.,Muench, S.,Munck, J.M.,Norton, D.,O'Reilly, M.,Palmer, N.,Pathuri, P.,Peakman, T.M.,Reader, M.,Rees, D.C.,Rich, S.J.,Shah, A.,Wallis, N.G.,Walton, H.,Wilsher, N.E.,Woolford, A.J.,Cooke, M.,Cousin, D.,Onions, S.,Shannon, J.,Watts, J.,Murray, C.W.
Discovery of ASTX029, A Clinical Candidate Which Modulates the Phosphorylation and Catalytic Activity of ERK1/2.
J.Med.Chem., 64:12286-12303, 2021

PubMed Abstract: Aberrant activation of the mitogen-activated protein kinase pathway frequently drives tumor growth, and the ERK1/2 kinases are positioned at a key node in this pathway, making them important targets for therapeutic intervention. Recently, a number of ERK1/2 inhibitors have been advanced to investigational clinical trials in patients with activating mutations in B-Raf proto-oncogene or Ras. Here, we describe the discovery of the clinical candidate ASTX029 () through structure-guided optimization of our previously published isoindolinone lead (). The medicinal chemistry campaign focused on addressing CYP3A4-mediated metabolism and maintaining favorable physicochemical properties. These efforts led to the identification of ASTX029, which showed the desired pharmacological profile combining ERK1/2 inhibition with suppression of phospho-ERK1/2 (pERK) levels, and in addition, it possesses suitable preclinical pharmacokinetic properties predictive of once daily dosing in humans. ASTX029 is currently in a phase I-II clinical trial in patients with advanced solid tumors.

PubMed: 34387469
DOI: 10.1021/acs.jmedchem.1c00905

Experimental method

X-RAY DIFFRACTION (1.897 Å)