7NIW

Nanodisc reconstituted human ABCB4 in complex with 4B1-Fab (posaconazole-bound, inward-open conformation)

7NIW の概要

• エントリーDOI: 10.2210/pdb7niw/pdb
• 関連するPDBエントリー: 7NIU 7NIV
• EMDBエントリー: 12367
• 分子名称: Phosphatidylcholine translocator ABCB4, 4B1 Fab-fragment light chain, 4B1 Fab-fragment heavy chain, ... (6 entities in total)
• 機能のキーワード: abcb4, mdr3, nanodisc, lipid transporter, transporter, phosphatidylcholine, protein transport
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 193737.72

構造登録者

Nosol, K.,Locher, K.P. (登録日: 2021-02-14, 公開日: 2021-08-25, 最終更新日: 2024-10-16)

主引用文献

Nosol, K.,Bang-Sorensen, R.,Irobalieva, R.N.,Erramilli, S.K.,Stieger, B.,Kossiakoff, A.A.,Locher, K.P.
Structures of ABCB4 provide insight into phosphatidylcholine translocation.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: ABCB4 is expressed in hepatocytes and translocates phosphatidylcholine into bile canaliculi. The mechanism of specific lipid recruitment from the canalicular membrane, which is essential to mitigate the cytotoxicity of bile salts, is poorly understood. We present cryogenic electron microscopy structures of human ABCB4 in three distinct functional conformations. An apo-inward structure reveals how phospholipid can be recruited from the inner leaflet of the membrane without flipping its orientation. An occluded structure reveals a single phospholipid molecule in a central cavity. Its choline moiety is stabilized by cation-π interactions with an essential tryptophan residue, rationalizing the specificity of ABCB4 for phosphatidylcholine. In an inhibitor-bound structure, a posaconazole molecule blocks phospholipids from reaching the central cavity. Using a proteoliposome-based translocation assay with fluorescently labeled phosphatidylcholine analogs, we recapitulated the substrate specificity of ABCB4 in vitro and confirmed the role of the key tryptophan residue. Our results provide a structural basis for understanding an essential translocation step in the generation of bile and its sensitivity to azole drugs.

PubMed: 34385322
DOI: 10.1073/pnas.2106702118

実験手法

ELECTRON MICROSCOPY (3.8 Å)