7NAF

State E2 nucleolar 60S ribosomal biogenesis intermediate - Spb1-MTD local model

7NAF の概要

• エントリーDOI: 10.2210/pdb7naf/pdb
• 関連するPDBエントリー: 7NAC 7NAD
• EMDBエントリー: 24269 24270 24271
• 分子名称: 25S rRNA, 60S ribosome ribosomal protein L19A, 60S ribosomal protein L30, ... (18 entities in total)
• 機能のキーワード: ribosome biogenesis, dead-box atpases, methyltransferase, nucleolus, ribosome
• 由来する生物種: Saccharomyces cerevisiae BY4741; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 311691.26

構造登録者

Cruz, V.E.,Sekulski, K.,Peddada, N.,Erzberger, J.P. (登録日: 2021-06-21, 公開日: 2022-11-09, 最終更新日: 2024-06-05)

主引用文献

Cruz, V.E.,Sekulski, K.,Peddada, N.,Sailer, C.,Balasubramanian, S.,Weirich, C.S.,Stengel, F.,Erzberger, J.P.
Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
Nat.Struct.Mol.Biol., 29:1228-1238, 2022

PubMed Abstract: DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular roles, most notably in driving large-scale RNA remodeling steps during the assembly of ribonucleoproteins (RNPs). We describe cryo-EM structures of 60S ribosomal biogenesis intermediates that reveal how context-specific RNA unwinding by the DEAD-box ATPase Spb4 results in extensive, sequence-specific remodeling of rRNA secondary structure. Multiple cis and trans interactions stabilize Spb4 in a post-catalytic, high-energy intermediate that drives the organization of the three-way junction at the base of rRNA domain IV. This mechanism explains how limited strand separation by DEAD-box ATPases is leveraged to provide non-equilibrium directionality and ensure efficient and accurate RNP assembly.

PubMed: 36482249
DOI: 10.1038/s41594-022-00874-9

実験手法

ELECTRON MICROSCOPY (3.13 Å)