7N77

Cryo-EM structure of ATP13A2 D458N/D962N mutant in the AlF-bound E1P-like state

7N77 の概要

• エントリーDOI: 10.2210/pdb7n77/pdb
• EMDBエントリー: 24212 24213 24214 24215 24216 24217 24218 24219 24220 24221 24222 24223
• 分子名称: Isoform 3 of Polyamine-transporting ATPase 13A2, TETRAFLUOROALUMINATE ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: p-type atpase, p5b-atpase, polyamine transporter, transport protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 129543.23

構造登録者

Sim, S.I.,Park, E. (登録日: 2021-06-09, 公開日: 2021-11-10, 最終更新日: 2025-05-28)

主引用文献

Sim, S.I.,von Bulow, S.,Hummer, G.,Park, E.
Structural basis of polyamine transport by human ATP13A2 (PARK9).
Mol.Cell, 81:4635-4649.e8, 2021

PubMed Abstract: Polyamines are small, organic polycations that are ubiquitous and essential to all forms of life. Currently, how polyamines are transported across membranes is not understood. Recent studies have suggested that ATP13A2 and its close homologs, collectively known as P5B-ATPases, are polyamine transporters at endo-/lysosomes. Loss-of-function mutations of ATP13A2 in humans cause hereditary early-onset Parkinson's disease. To understand the polyamine transport mechanism of ATP13A2, we determined high-resolution cryoelectron microscopy (cryo-EM) structures of human ATP13A2 in five distinct conformational intermediates, which together, represent a near-complete transport cycle of ATP13A2. The structural basis of the polyamine specificity was revealed by an endogenous polyamine molecule bound to a narrow, elongated cavity within the transmembrane domain. The structures show an atypical transport path for a water-soluble substrate, in which polyamines may exit within the cytosolic leaflet of the membrane. Our study provides important mechanistic insights into polyamine transport and a framework to understand the functions and mechanisms of P5B-ATPases.

PubMed: 34715013
DOI: 10.1016/j.molcel.2021.08.017

実験手法

ELECTRON MICROSCOPY (3.2 Å)