7N62

SARS-CoV-2 Spike (2P) in complex with C12C9 Fab (NTD local reconstruction)

Summary for 7N62

• Entry DOI: 10.2210/pdb7n62/pdb
• EMDB information: 24192
• Descriptor: Spike glycoprotein, C12C9 Fab heavy chain, C12C9 Fab light chain, ... (4 entities in total)
• Functional Keywords: coronavirus, antibody, epitope, viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); More
• Total number of polymer chains: 3
• Total formula weight: 190195.66

Authors

Windsor, I.W.,Jenni, S.,Bajic, G.,Tong, P.,Gautam, A.K.,Wesemann, D.R.,Harrison, S.C. (deposition date: 2021-06-07, release date: 2021-08-04, Last modification date: 2024-11-13)

Primary citation

Tong, P.,Gautam, A.,Windsor, I.,Travers, M.,Chen, Y.,Garcia, N.,Whiteman, N.B.,McKay, L.G.A.,Lelis, F.J.N.,Habibi, S.,Cai, Y.,Rennick, L.J.,Duprex, W.P.,McCarthy, K.R.,Lavine, C.L.,Zuo, T.,Lin, J.,Zuiani, A.,Feldman, J.,MacDonald, E.A.,Hauser, B.M.,Griffths, A.,Seaman, M.S.,Schmidt, A.G.,Chen, B.,Neuberg, D.,Bajic, G.,Harrison, S.C.,Wesemann, D.R.
Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike.
Biorxiv, 2021

PubMed Abstract: Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.

PubMed: 33758863
DOI: 10.1101/2021.03.10.434840

Experimental method

ELECTRON MICROSCOPY (4 Å)