7N59
Structure of AtAtm3 in the inward-facing conformation with GSSG bound
Summary for 7N59
| Entry DOI | 10.2210/pdb7n59/pdb |
| EMDB information | 24183 |
| Descriptor | ABC transporter B family member 25, mitochondrial, OXIDIZED GLUTATHIONE DISULFIDE (2 entities in total) |
| Functional Keywords | atpase, membrane protein |
| Biological source | Arabidopsis thaliana (Mouse-ear cress) |
| Total number of polymer chains | 2 |
| Total formula weight | 164199.32 |
| Authors | Fan, C.,Rees, D.C. (deposition date: 2021-06-05, release date: 2022-04-13, Last modification date: 2024-05-29) |
| Primary citation | Fan, C.,Rees, D.C. Glutathione binding to the plant At Atm3 transporter and implications for the conformational coupling of ABC transporters. Elife, 11:-, 2022 Cited by PubMed Abstract: The ATP binding cassette (ABC) transporter of mitochondria (Atm) from (Atm3) has been implicated in the maturation of cytosolic iron-sulfur proteins and heavy metal detoxification, plausibly by exporting glutathione derivatives. Using single-particle cryo-electron microscopy, we have determined four structures of Atm3 in three different conformational states: two inward-facing conformations (with and without bound oxidized glutathione [GSSG]), together with closed and outward-facing states stabilized by MgADP-VO. These structures not only provide a structural framework for defining the alternating access transport cycle, but also reveal the paucity of cysteine residues in the glutathione binding site that could potentially form inhibitory mixed disulfides with GSSG. Despite extensive efforts, we were unable to prepare the ternary complex of Atm3 containing both GSSG and MgATP. A survey of structurally characterized type IV ABC transporters that includes Atm3 establishes that while nucleotides are found associated with all conformational states, they are effectively required to stabilize occluded, closed, and outward-facing conformations. In contrast, transport substrates have only been observed associated with inward-facing conformations. The absence of structures with dimerized nucleotide binding domains containing both nucleotide and transport substrate suggests that this form of the ternary complex exists only transiently during the transport cycle. PubMed: 35333177DOI: 10.7554/eLife.76140 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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