7N19

DR3 in complex with Aspergillus nidulans NAD-dependent histone deacetylase hst4 peptide

Summary for 7N19

• Entry DOI: 10.2210/pdb7n19/pdb
• Descriptor: HLA class II histocompatibility antigen, DR alpha chain, HLA class II histocompatibility antigen DR beta chain, HST4 peptide, ... (7 entities in total)
• Functional Keywords: mhcii, t cell, immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 12
• Total formula weight: 182487.73

Authors

Wang, Y.,Dai, S. (deposition date: 2021-05-27, release date: 2021-08-18, Last modification date: 2024-11-06)

Primary citation

Greaves, S.A.,Ravindran, A.,Santos, R.G.,Chen, L.,Falta, M.T.,Wang, Y.,Mitchell, A.M.,Atif, S.M.,Mack, D.G.,Tinega, A.N.,Maier, L.A.,Dai, S.,Pinilla, C.,Grunewald, J.,Fontenot, A.P.
CD4+ T cells in the lungs of acute sarcoidosis patients recognize an Aspergillus nidulans epitope.
J.Exp.Med., 218:-, 2021

PubMed Abstract: Löfgren's syndrome (LS) is an acute form of sarcoidosis characterized by a genetic association with HLA-DRB1*03 (HLA-DR3) and an accumulation of CD4+ T cells of unknown specificity in the bronchoalveolar lavage (BAL). Here, we screened related LS-specific TCRs for antigen specificity and identified a peptide derived from NAD-dependent histone deacetylase hst4 (NDPD) of Aspergillus nidulans that stimulated these CD4+ T cells in an HLA-DR3-restricted manner. Using ELISPOT analysis, a greater number of IFN-γ- and IL-2-secreting T cells in the BAL of DR3+ LS subjects compared with DR3+ control subjects was observed in response to the NDPD peptide. Finally, increased IgG antibody responses to A. nidulans NDPD were detected in the serum of DR3+ LS subjects. Thus, our findings identify a ligand for CD4+ T cells derived from the lungs of LS patients and suggest a role of A. nidulans in the etiology of LS.

PubMed: 34410304
DOI: 10.1084/jem.20210785

Experimental method

X-RAY DIFFRACTION (2.38 Å)