7N0C

Cryo-EM structure of the monomeric form of SARS-CoV-2 nsp10-nsp14 (E191A)-RNA complex

7N0C の概要

• エントリーDOI: 10.2210/pdb7n0c/pdb
• EMDBエントリー: 24103
• 分子名称: Non-structural protein 10, Proofreading exoribonuclease, RNA (25-MER), ... (7 entities in total)
• 機能のキーワード: sars-cov-2, exoribonuclease, mismatch correction, viral protein-rna complex, viral protein/rna
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97368.31

構造登録者

Liu, C.,Yang, Y. (登録日: 2021-05-25, 公開日: 2021-07-28, 最終更新日: 2025-05-28)

主引用文献

Liu, C.,Shi, W.,Becker, S.T.,Schatz, D.G.,Liu, B.,Yang, Y.
Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme.
Science, 373:1142-1146, 2021

PubMed Abstract: Coronavirus 3′-to-5′ exoribonuclease (ExoN), residing in the nonstructural protein (nsp) 10–nsp14 complex, boosts replication fidelity by proofreading RNA synthesis and is critical for the virus life cycle. ExoN also recognizes and excises nucleotide analog inhibitors incorporated into the nascent RNA, undermining the effectiveness of nucleotide analog–based antivirals. Here we present cryo–electron microscopy structures of both wild-type and mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nsp10-nsp14 in complex with an RNA substrate bearing a 3′-end mismatch at resolutions ranging from 2.5 to 3.9 angstroms. The structures reveal the molecular determinants of ExoN substrate specificity and offer insight into the molecular mechanisms of mismatch correction during coronavirus RNA synthesis. Our findings provide guidance for rational design of improved anticoronavirus therapies.

PubMed: 34315827
DOI: 10.1126/science.abi9310

実験手法

ELECTRON MICROSCOPY (3.4 Å)