7MWE

HUWE1 in map with focus on WWE

7MWE の概要

• エントリーDOI: 10.2210/pdb7mwe/pdb
• 関連するPDBエントリー: 7JQ9 7MOP
• EMDBエントリー: 22427 22428 22429 22430 22431 23925 23931
• 分子名称: E3 ubiquitin-protein ligase HUWE1 (1 entity in total)
• 機能のキーワード: ubiquitin, quality control, e3 ligase, protein degradation, transferase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 486409.53

構造登録者

Hunkeler, M.,Fischer, E.S. (登録日: 2021-05-16, 公開日: 2021-07-28, 最終更新日: 2024-05-29)

主引用文献

Hunkeler, M.,Jin, C.Y.,Ma, M.W.,Monda, J.K.,Overwijn, D.,Bennett, E.J.,Fischer, E.S.
Solenoid architecture of HUWE1 contributes to ligase activity and substrate recognition.
Mol.Cell, 81:3468-, 2021

PubMed Abstract: HECT ubiquitin ligases play essential roles in metazoan development and physiology. The HECT ligase HUWE1 is central to the cellular stress response by mediating degradation of key death or survival factors, including Mcl1, p53, DDIT4, and Myc. Although mutations in HUWE1 and related HECT ligases are widely implicated in human disease, our molecular understanding remains limited. Here we present a comprehensive investigation of full-length HUWE1, deepening our understanding of this class of enzymes. The N-terminal ∼3,900 amino acids of HUWE1 are indispensable for proper ligase function, and our cryo-EM structures of HUWE1 offer a complete molecular picture of this large HECT ubiquitin ligase. HUWE1 forms an alpha solenoid-shaped assembly with a central pore decorated with protein interaction modules. Structures of HUWE1 variants linked to neurodevelopmental disorders as well as of HUWE1 bound to a model substrate link the functions of this essential enzyme to its three-dimensional organization.

PubMed: 34314700
DOI: 10.1016/j.molcel.2021.06.032

実験手法

ELECTRON MICROSCOPY (3.4 Å)