7MLS
X-ray crystal structure of human BRD4(D1) in complex with 2-(2,5-dibromophenoxy)-6-[4-methyl-1-(piperidin-4-yl)-1H-1,2,3-triazol-5-yl]pyridine (compound 23)
Summary for 7MLS
| Entry DOI | 10.2210/pdb7mls/pdb |
| Descriptor | Bromodomain-containing protein 4, 2-(2,5-dibromophenoxy)-6-[4-methyl-1-(piperidin-4-yl)-1H-1,2,3-triazol-5-yl]pyridine, 1,2-ETHANEDIOL, ... (5 entities in total) |
| Functional Keywords | brd4, gene regulation, gene regulation-inhibitor complex, gene regulation/inhibitor |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 15787.62 |
| Authors | Cui, H.,Johnson, J.A.,Zahid, H.,Buchholz, C.R.,Shi, K.,Aihara, H.,Pomerantz, W.C.K. (deposition date: 2021-04-28, release date: 2021-07-28, Last modification date: 2023-10-18) |
| Primary citation | Cui, H.,Carlson, A.S.,Schleiff, M.A.,Divakaran, A.,Johnson, J.A.,Buchholz, C.R.,Zahid, H.,Vail, N.R.,Shi, K.,Aihara, H.,Harki, D.A.,Miller, G.P.,Topczewski, J.J.,Pomerantz, W.C.K. 4-Methyl-1,2,3-Triazoles as N -Acetyl-Lysine Mimics Afford Potent BET Bromodomain Inhibitors with Improved Selectivity. J.Med.Chem., 64:10497-10511, 2021 Cited by PubMed Abstract: The bromodomain and extra terminal (BET) protein family recognizes acetylated lysines within histones and transcription factors using two N-terminal bromodomains, D1 and D2. The protein-protein interactions between BET bromodomains, acetylated histones, and transcription factors are therapeutic targets for BET-related diseases, including inflammatory disease and cancer. Prior work demonstrated that methylated-1,2,3-triazoles are suitable -acetyl lysine mimetics for BET inhibition. Here we describe a structure-activity relationship study of triazole-based inhibitors that improve affinity, D1 selectivity, and microsomal stability. These outcomes were accomplished by targeting a nonconserved residue, Asp144 and a conserved residue, Met149, on BRD4 D1. The lead inhibitors and have a BRD4 D1 of 12 and 6.4 nM, respectively. Cellular activity was demonstrated through suppression of c-Myc expression in MM.1S cells and downregulation of IL-8 in TNF-α-stimulated A549 cells. These data indicate that and are new leads to investigate the anticancer and anti-inflammatory activity of BET proteins. PubMed: 34236185DOI: 10.1021/acs.jmedchem.1c00933 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.26 Å) |
Structure validation
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