7MKK

Crystal structure of Drosophila Panoramix in complex with Sov NTD

7MKK の概要

• エントリーDOI: 10.2210/pdb7mkk/pdb
• 分子名称: Small ovary, isoform A, Protein panoramix (3 entities in total)
• 機能のキーワード: piwi, transposon silencing, heterochromatin formation, pirna pathway, transcriptional silencing, rna binding-metal binding protein complex, rna binding/metal binding protein
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 49413.24

構造登録者

Wang, J.,Patel, D.J. (登録日: 2021-04-24, 公開日: 2022-02-02, 最終更新日: 2024-05-22)

主引用文献

Andreev, V.I.,Yu, C.,Wang, J.,Schnabl, J.,Tirian, L.,Gehre, M.,Handler, D.,Duchek, P.,Novatchkova, M.,Baumgartner, L.,Meixner, K.,Sienski, G.,Patel, D.J.,Brennecke, J.
Panoramix SUMOylation on chromatin connects the piRNA pathway to the cellular heterochromatin machinery.
Nat.Struct.Mol.Biol., 29:130-142, 2022

PubMed Abstract: Nuclear Argonaute proteins, guided by small RNAs, mediate sequence-specific heterochromatin formation. The molecular principles that link Argonaute-small RNA complexes to cellular heterochromatin effectors on binding to nascent target RNAs are poorly understood. Here, we explain the mechanism by which the PIWI-interacting RNA (piRNA) pathway connects to the heterochromatin machinery in Drosophila. We find that Panoramix, a corepressor required for piRNA-guided heterochromatin formation, is SUMOylated on chromatin in a Piwi-dependent manner. SUMOylation, together with an amphipathic LxxLL motif in Panoramix's intrinsically disordered repressor domain, are necessary and sufficient to recruit Small ovary (Sov), a multi-zinc-finger protein essential for general heterochromatin formation and viability. Structure-guided mutations that eliminate the Panoramix-Sov interaction or that prevent SUMOylation of Panoramix uncouple Sov from the piRNA pathway, resulting in viable but sterile flies in which Piwi-targeted transposons are derepressed. Thus, Piwi engages the heterochromatin machinery specifically at transposon loci by coupling recruitment of a corepressor to nascent transcripts with its SUMOylation.

PubMed: 35173350
DOI: 10.1038/s41594-022-00721-x

実験手法

X-RAY DIFFRACTION (2.5 Å)