7MJL
Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to Fab ab1 (focused refinement of RBD and Fab ab1)
Summary for 7MJL
| Entry DOI | 10.2210/pdb7mjl/pdb |
| EMDB information | 23877 |
| Descriptor | Spike glycoprotein, Fab ab1 Heavy Chain, Fab ab1 Light Chain, ... (4 entities in total) |
| Functional Keywords | sars-cov-2, glycoprotein, fusion protein, viral protein, fab ab1, neutralizing antibody, viral protein-immune system complex, viral protein/immune system |
| Biological source | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2) More |
| Total number of polymer chains | 3 |
| Total formula weight | 191977.28 |
| Authors | Zhu, X.,Mannar, D.,Srivastava, S.S.,Berezuk, A.M.,Demers, J.P.,Saville, J.W.,Leopold, K.,Li, W.,Dimitrov, D.S.,Tuttle, K.S.,Zhou, S.,Chittori, S.,Subramaniam, S. (deposition date: 2021-04-20, release date: 2021-05-12, Last modification date: 2024-10-09) |
| Primary citation | Zhu, X.,Mannar, D.,Srivastava, S.S.,Berezuk, A.M.,Demers, J.P.,Saville, J.W.,Leopold, K.,Li, W.,Dimitrov, D.S.,Tuttle, K.S.,Zhou, S.,Chittori, S.,Subramaniam, S. Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies. Plos Biol., 19:e3001237-e3001237, 2021 Cited by PubMed Abstract: The recently reported "UK variant" (B.1.1.7) of SARS-CoV-2 is thought to be more infectious than previously circulating strains as a result of several changes, including the N501Y mutation. We present a 2.9-Å resolution cryo-electron microscopy (cryo-EM) structure of the complex between the ACE2 receptor and N501Y spike protein ectodomains that shows Y501 inserted into a cavity at the binding interface near Y41 of ACE2. This additional interaction provides a structural explanation for the increased ACE2 affinity of the N501Y mutant, and likely contributes to its increased infectivity. However, this mutation does not result in large structural changes, enabling important neutralization epitopes to be retained in the spike receptor binding domain. We confirmed this through biophysical assays and by determining cryo-EM structures of spike protein ectodomains bound to 2 representative potent neutralizing antibody fragments. PubMed: 33914735DOI: 10.1371/journal.pbio.3001237 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.95 Å) |
Structure validation
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