7M8J

SARS-CoV-2 S-NTD + Fab CM25

Summary for 7M8J

• Entry DOI: 10.2210/pdb7m8j/pdb
• EMDB information: 23717
• Descriptor: Spike protein S1, CM25 Fab - Heavy Chain, CM25 Fab - Light Chain (3 entities in total)
• Functional Keywords: sars-cov-2, cm25, ntd, fab, viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); More
• Total number of polymer chains: 3
• Total formula weight: 53770.21

Authors

Johnson, N.V.,Mclellan, J.S. (deposition date: 2021-03-29, release date: 2021-05-19, Last modification date: 2024-11-06)

Primary citation

Voss, W.N.,Hou, Y.J.,Johnson, N.V.,Delidakis, G.,Kim, J.E.,Javanmardi, K.,Horton, A.P.,Bartzoka, F.,Paresi, C.J.,Tanno, Y.,Chou, C.W.,Abbasi, S.A.,Pickens, W.,George, K.,Boutz, D.R.,Towers, D.M.,McDaniel, J.R.,Billick, D.,Goike, J.,Rowe, L.,Batra, D.,Pohl, J.,Lee, J.,Gangappa, S.,Sambhara, S.,Gadush, M.,Wang, N.,Person, M.D.,Iverson, B.L.,Gollihar, J.D.,Dye, J.M.,Herbert, A.S.,Finkelstein, I.J.,Baric, R.S.,McLellan, J.S.,Georgiou, G.,Lavinder, J.J.,Ippolito, G.C.
Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes.
Science, 372:1108-1112, 2021

PubMed Abstract: The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are unknown. Proteomic deconvolution of the IgG repertoire to the spike glycoprotein in convalescent subjects revealed that the response is directed predominantly (>80%) against epitopes residing outside the receptor binding domain (RBD). In one subject, just four IgG lineages accounted for 93.5% of the response, including an amino (N)-terminal domain (NTD)-directed antibody that was protective against lethal viral challenge. Genetic, structural, and functional characterization of a multidonor class of "public" antibodies revealed an NTD epitope that is recurrently mutated among emerging SARS-CoV-2 variants of concern. These data show that "public" NTD-directed and other non-RBD plasma antibodies are prevalent and have implications for SARS-CoV-2 protection and antibody escape.

PubMed: 33947773
DOI: 10.1126/science.abg5268

Experimental method

ELECTRON MICROSCOPY (3.48 Å)