7M5O

Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA

Summary for 7M5O

• Entry DOI: 10.2210/pdb7m5o/pdb
• EMDB information: 23678
• Descriptor: CasPhi, crRNA, ZINC ION (3 entities in total)
• Functional Keywords: crispr, casphi, cas12j, nuclease, crrna, rnp, complex, viral protein-rna complex, viral protein/rna
• Biological source: Biggievirus Mos11 (Biggievirus); More
• Total number of polymer chains: 2
• Total formula weight: 100674.25

Authors

Pausch, P.,Soczek, K.,Nogales, E.,Doudna, J. (deposition date: 2021-03-24, release date: 2021-08-04, Last modification date: 2021-08-25)

• Primary citation: Pausch, P.,Soczek, K.M.,Herbst, D.A.,Tsuchida, C.A.,Al-Shayeb, B.,Banfield, J.F.,Nogales, E.,Doudna, J.A.; DNA interference states of the hypercompact CRISPR-Cas Phi effector.; Nat.Struct.Mol.Biol., 28:652-661, 2021; PubMed: 34381246; DOI: 10.1038/s41594-021-00632-3

Experimental method

ELECTRON MICROSCOPY (3.54 Å)